Bunga Harum Sundal Malam

Perennial  and night blooming plant. It grows in elongated spikes up to 45 cm long that produce clusters of fragrant waxy white flowers that bloom from the bottom towards the top of the spike.

Sapogenin as antipyretics and eliminate swelling. The single floweed tuberose has high essential content ranging 0.08-0.10%.

The flower volatile compounds were identified as benzyl benzoate, methyl (E)-isoeugenol, ethyl myristate, and methyl anthranilate (Mimaki Y.
et al
2002).

Several steroid sapogenins, such as hecogenin, 9-dehydroxyhecogenin, and tigogenin, as well as glycosides, 29-hydroxystigmast-5-en-3β-yl β-Dglucoside,

(22S)-2β,3β,22-trihydroxycholest-5-en-16β-yl β-Dglucoside, and diribo furano-sylethyl eneglycol, and spirostanolpentaglycosides  were identified from the underground parts of
P. tuberosa. Our search for bioactive saponins from

P. tuberosa


has led to the isolation of six new steroid glycosidess two spirostanols, polianthosides B and C (1,
2), and four furostanols, polianthosides D-G (3–6) together with eight known saponins (7–14) from the fresh tubers. (Jin J.M. et al 2004).

A few glycosides, such as (22S)-2β,3β,22- trihydroxycholest-5-en-16β-yl β-D-apiofuranoside, 29-hydroxystigmast- 5-en-3β-yl β-D-glucopyranoside, and diribofuranosyl ethyleneglycol, were isolated from the underground parts of
P. tuberosa. Cholestan glycoside and three  steroidal saponins isolated from the aerial parts of
P. tuberosa

Rheumatoid arthritis:

30 gm root, 20 gm aplina purpurata, dark brown sugar, 400cc water, boil. Strain and 2 parts to be used twice a day.

Polianthes tuberosa


Saponin 7

Four spirostanol saponins with five monosaccharides (1-4). The cytotoxic activities of  against HL-60 human prolyelocytic leukimia cells and HSC-2 human oral sequacous cell carninoma cells (Mimaki, Y.,
et al., 2002).

Although the cytotoxicity of against HL-60 cells was moderate (IC₅₀
3.9-9.0 µg/mL) compared with that of etoposide used as a positive control (IC₅₀
0.30 µg/mL),

HSC-2 cells, which were resistant to etoposide (IC₅₀
24.4 µg/mL), were relatively sensitive to the four spirostanol, showing IC₅₀
values ranging between 1.5 and 13.0 µg/mL (Mimaki, Y.,
et al., 2002).

Modulatory effect on the murine self defense system

Modulatory effect on the murine self defense system by a newly discovered acidic polysaccharide (ANK-102) produced by
P. tuberosa
cells in liquid culture was examined. Pretreatment with ANK-102 deteriorated the murine survival against lethal infection of Listeria monocytogenes, an intracellular gram-positive bacterium eliminated mainly by macrophages through Falak-cell mediated immune response. Pretreatment with ANK-102 resulted in the accumulation of Mac 1 and Mac 2 positive cells in the peritoneal cavity of the infected animals and the reduction of Thy1.2 expression on the surface of the thymocytes. (Majima T., 1995).

Effects on snail

The powder of
P. tuberosa
bulb and its sapogenins tigogenin and hecogenin on the reproduction of the snail
Lymnaea acuminata. It has been reported that the two sapogenins tigogenin and hecogenin caused a significant reduction in fecundity, hatchbility , and survival of young snails. Exposure to sub-lethal concentration caused a small but significant reduction in proteins, amino acids, nucleic acids and phosphtase activitites in gonadal tissue of treated sanils (Singh K
et al
1999).

Jin J.M., Zhang Y.J. and Yang C.R. (2004). Spirostanol and Furostanol Glycosides from the Fresh Tubers of Polianthes tuberosa.
J. Nat Prod
; 67 pg 5-9.

http://nieare11.blogspot.com/2012/02/various-blossoms-polianthes-tuberosa.html

Majima N., K. Otsuji, R. Nagatomi, Konno T. (1995) Polysaccharide (ANK0102) from Polianthes tuberosa cells deterorates the resistance of mice to Listeria monocytorenes infection.
http://www.researchgate.net/researcher/68170940_T_Majima/

Mimaki Y.’, A. Yokosuka, C. Sakuma, H. Sakagami and Y. Sashida,
J. Nat. Prod.,
65, 1424 (2002).

http://download.springer.com/static/pdf/

Singh K., V.K. Singh, Singh D.K. (1999) Effect of
Polianthes tuberosa
(Amaryllidaceae) on the Reproduction and Biochemical Parameters in the Ovotestis of Snail
Lymnaea acuminata
(Mollusca, Pulmonata).
Acta hydrochimica et hydrobiologica. Vol 27 (1) pg 32-37.